Unlocking the power of Zc3h12c: Orchestrating Macrophage activation and elevating the innate immune response

• Published in: Cellular immunology Vol. 401-402; p. 104837
• Main Authors: Zhao, Yinxia, Zhu, Maoli, Wu, Songfang, Ou, Meixian, Xi, Yang, Liu, Zhen, Hu, Rui, Li, Xiaowei, Xu, Ting, Xiang, Xiaoqing, Zhou, Ying, Li, Shuijun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.07.2024
• Subjects: Animals; Humans; Immunity, Innate - immunology; Interleukin-6 - immunology; Interleukin-6 - metabolism; Lipopolysaccharides - immunology; Lipopolysaccharides - pharmacology; Macrophage activation; Macrophage Activation - immunology; Macrophages - immunology; Macrophages - metabolism; Mice; NF-kappa B - metabolism; RAW 264.7 Cells; Signal Transduction - immunology; Toll-like receptors; Toll-Like Receptors - immunology; Toll-Like Receptors - metabolism; Tumor Necrosis Factor-alpha - immunology; Tumor Necrosis Factor-alpha - metabolism; Zc3h12c; Toll-like receptors; Macrophage activation; Zc3h12c
• ISSN: 0008-8749; 1090-2163; 1090-2163
• DOI: 10.1016/j.cellimm.2024.104837

•Zc3h12c is induced in macrophages following stimulation with TLR agonists and pathogens.•Zc3h12c significantly mitigated the release of TNF-α and IL-6 triggered by lipopolysaccharide.•Zc3h12c exerted a notable inhibitory effect on JNK, ERK, p38, and NF-κB signaling. The activation of macrophages, essential for the innate defense against invading pathogens, revolves around Toll-like receptors (TLRs). Nevertheless, a comprehensive understanding of the molecular mechanisms governing TLR signaling in the course of macrophage activation remains to be fully clarified. Although Zc3h12c was originally identified as being enriched in organs associated with macrophages, its precise function remains elusive. In this study, we observed a significant induction of Zc3h12c in macrophages following stimulation with TLR agonists and pathogens. Overexpression of Zc3h12c significantly mitigated the release of TNF-α and IL-6 triggered by lipopolysaccharide (LPS), whereas depletion of Zc3h12c increased the production of the cytokines mentioned above. Notably, the expression of IFN-β was not influenced by Zc3h12c. Luciferase reporter assays revealed that Zc3h12c could suppress the TNF-α promoter activity. Moreover, Zc3h12c exerted a notable inhibitory effect on JNK, ERK, p38, and NF-κB signaling induced by LPS. In summary, the findings of our study suggest that Zc3h12c functions as a robust suppressor of innate immunity, potentially playing a role in the pathogenesis of infectious diseases.