Hematological, biochemical, genotoxic, and histopathological changes induced by pyridaben

The current work examined the genotoxic effects of pyridaben (PDB) in male Sprague Dawley rats. Twenty Sprague Dawley rats were divided into four equal groups; the first group was used as a control group; the other three groups were exposed to 19, 28.5, and 57 mg/kg b.w PDB by oral gavage for 4 week...

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Bibliographic Details
Published inEnvironmental toxicology Vol. 38; no. 10; pp. 2391 - 2399
Main Authors Ali, Marwa F, Mohamed, Wafaa H
Format Journal Article
LanguageEnglish
Published United States Wiley Subscription Services, Inc 01.10.2023
Subjects
Alkaline phosphatase
Aspartate aminotransferase
Bone marrow
Chromosome aberrations
Chromosomes
Exposure
Fibrosis
Gene expression
Genotoxicity
Glutathione
Glutathione peroxidase
Hematology
Histopathology
Inflammation
Interleukin 6
Liver
Parameters
Peroxidase
Phosphatase
Pyridaben
genotoxicity
chromosomal aberrations
histopathological examination
immunhistochemical investigation
pyridaben
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Summary:The current work examined the genotoxic effects of pyridaben (PDB) in male Sprague Dawley rats. Twenty Sprague Dawley rats were divided into four equal groups; the first group was used as a control group; the other three groups were exposed to 19, 28.5, and 57 mg/kg b.w PDB by oral gavage for 4 weeks. Blood samples were collected for hematological and biochemical parameters; femoral bone marrow was flushed for chromosomal aberrations (CA) assay and liver samples were used for the analysis of gene expression of IL-6 and Casp-3 as well as histopathological and immunhistochemical investigation for Casp-3. The results showed that PDB exposure lead to non-significant changes in hematological parameters in all PDB administrated groups while malondialdehyde, glutathione peroxidase, aspartate aminotransferase, and alkaline phosphatase were significantly increased in 19 and 57 mg/kg PDB doses groups Also, gene expression of IL-6 and Casp-3 revealed a significant increase in 28.5 and 57 mg/kg PDB doses groups as compared with the control. However, there was no significant change in the percentage of CAs in bone marrow cells in all PDB-exposed groups. The histopathological and immunhistochemical examination showed focal areas of inflammatory cellular infiltration with fibrosis in 57 mg/kg b.w PDB dose group accompanied by the severe positive reaction of caspase3 in the liver.
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ISSN:1520-4081
1522-7278
DOI:10.1002/tox.23875