Enhanced productivity of Protein C by recombinant human cells in automated fed-batch cultures

Recombinant human kidney cells, 293, were cultivated in serum-free fed-batch cultures for the production of Protein C. By coupling the feeding of concentrated medium to pH control based on established stoichiometric relations, the titer of Protein C increased by more than ten fold as compared to bat...

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Bibliographic Details
Published inCytotechnology (Dordrecht) Vol. 17; no. 2; p. 109
Main Authors Kyung, Y S, Hu, W S
Format Journal Article
LanguageEnglish
Published United States 01.01.1995
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Summary:Recombinant human kidney cells, 293, were cultivated in serum-free fed-batch cultures for the production of Protein C. By coupling the feeding of concentrated medium to pH control based on established stoichiometric relations, the titer of Protein C increased by more than ten fold as compared to batch culture, even though the total cell concentration increased only by less than two fold. Such a fed-batch culture is a simple system for enhancing the productivity of mammalian cells in culture.
ISSN:0920-9069
DOI:10.1007/BF00749398