Differentiation of neotropical fish species with statistical analysis of fourier transform infrared photoacoustic spectroscopy data

Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) was applied to nineteen fish species in Brazil's Upper Paraná River basin to identify differences in the structural composition of their scales. To differentiate the species, a canonical discriminant analysis was used to indicate...

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Bibliographic Details
Published inApplied spectroscopy Vol. 66; no. 7; p. 782
Main Authors Almeida, Francylaine S, Lima, Sandro M, Andrade, Luis H C, Súarez, Yzel R
Format Journal Article
LanguageEnglish
Published United States 01.07.2012
Subjects
Animals
Biomarkers - chemistry
Brazil
Discriminant Analysis
Fishes - classification
Fishes - genetics
Photoacoustic Techniques - methods
Phylogeny
Skin - chemistry
Spectroscopy, Fourier Transform Infrared - methods
Brazil
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Summary:Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) was applied to nineteen fish species in Brazil's Upper Paraná River basin to identify differences in the structural composition of their scales. To differentiate the species, a canonical discriminant analysis was used to indicate the most important absorption peaks in the mid-infrared region. Significant differences were found in the chemical composition of scales among the studied fish species, with Wilk's lambda = 5.2 × 10(-6), F((13,18,394)) = 37.57, and P < 0.001, indicating that O-CH(2) wag at 1396 cm(-1) can be used as a biomarker of this species group. The species could be categorized into four groups according to phylogenetic similarity, suggesting that the O-CH(2) 1396 cm(-1) absorbance is related to the biological traits of each species. This procedure can also be used to complement evolutionary studies.
ISSN:1943-3530
DOI:10.1366/11-06487