Production of swine pepsinogen by protein-producing Bacillus brevis carrying swine pepsinogen cDNA

• Published in: Applied microbiology and biotechnology Vol. 30; no. 1; pp. 75 - 80
• Main Authors: Takao, M, Morioka, T, Yamagata, H, Tsukagoshi, N, Udaka, S
• Format: Journal Article
• Language: English
• Published: 1989
• Subjects: Brevibacillus brevis; DNA; pepsinogen; production; proteins; swine
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/BF00256000

An expression-secretion vector, pNU100, was constructed, utilizing the promoter and coding sequences for the signal peptide and nine amino-terminal amino acids of the middle wall protein, to produce foreign protein by protein-producing Bacillus brevis. Expression of swine pepsinogen cDNA in B. brevis was examined with pNU100 as a vector. The recombinant swine pepsinogen synthesized by B. brevis was found to accumulate extracellularly in the form of a soluble protein and to have acid protease activity. The acid protease activity was completely inhibited by pepstatin. Furthermore, the recombinant pepsinogen was converted autocatalytically to pepsin under acidic conditions. This indicates that B. brevis produces a pepsinogen with the same conformation as authentic pepsinogen. Efficient production of the enzyme (11 mg/1) was achieved by regulating the pH of the medium. The enzyme produced by B. brevis remained stable on cultivation for a long period, up to 40 h. This is suggested to be due to a unique property of protein-producing B. brevis, i.e. a deficiency in extracellular protease production.