Micropropagation of recalcitrant-seeded Ekebergia capensis Sparrm. using in vitro nodal segments and roots

The present study reports the development of in vitro micropropagation protocols for Ekebergia capensis Sparrm., a species that produces unstorable recalcitrant (non-orthodox) seeds. Suitable protocols were established for shoots generated from nodal segments excised from in vitro-germinated seedlin...

Full description

Saved in:
Bibliographic Details
Published inThe journal of horticultural science & biotechnology Vol. 84; no. 1; pp. 87 - 91
Main Authors Hajari, E., Berjak, P., Pammenter, N.W., Watt, M.P.
Format Journal Article
LanguageEnglish
Published Taylor & Francis 01.01.2009
Online AccessGet full text

Cover

More Information
Summary:The present study reports the development of in vitro micropropagation protocols for Ekebergia capensis Sparrm., a species that produces unstorable recalcitrant (non-orthodox) seeds. Suitable protocols were established for shoots generated from nodal segments excised from in vitro-germinated seedlings, and for adventitious shoots generated from intact in vitro-germinated roots. Difficulties were experienced when attempts were made to micropropagate germplasm from established saplings. The protocols developed have important applications for the conservation (cryopreservation and clonal multiplication) of germplasm of this species.
ISSN:1462-0316
2380-4084
DOI:10.1080/14620316.2009.11512485