Prevalence of natural infection by Trypanosoma evansi in Crioula LAGEANA cattle

• Published in: Microbial pathogenesis Vol. 180; p. 106143
• Main Authors: Fiorin, Felipe Eduardo, Casa, Mariana da Silva, Griebeler, Leonardo Bergman, Goedel, Mariana Fuchs, Nascimento, Luís Flávio Nepomuceno do, Neves, Gabriella Bassi das, Fonteque, Graziela Vieira, Miletti, Luiz Claudio, Saito, Mere Erika, Fonteque, Joandes Henrique
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.07.2023
• Subjects: Animal health; Animals; Cattle; Cattle Diseases - epidemiology; DNA, Protozoan - genetics; Hematology; Locally adapted breed; Polymerase Chain Reaction - methods; Prevalence; Trypanosoma - genetics; Trypanosomiasis; Trypanosomiasis - epidemiology; Trypanosomiasis - parasitology; Trypanosomiasis - veterinary; Hematology; Locally adapted breed; Animal health; Trypanosomiasis
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1016/j.micpath.2023.106143

Cattle trypanosomiasis negatively impacts animal husbandry due to high morbidity, productivity losses, and mortality rates. Knowledge regarding Trypanosoma evansi infections in locally adapted breeds remains limited. Some cattle breeds exhibit trypanotolerance, requiring the determination of prevalence, as well as related tolerance and resistance characteristics, for disease control programs. This study aimed to determine T. evansi prevalence in Crioula Lageana cattle and associate clinical, hematological, and biochemical aspects with the infection to further research on tolerance in this population. Blood samples from 310 Crioula Lageana cattle were tested using Polymerase Chain Reaction (PCR) and Indirect Immunofluorescence Reaction (IIFR). T. evansi prevalence was 8% (24/310) using PCR and 4% (11/310) using IIFR. Positive animals showed increased ruminal movements, elevated eosinophil counts, and reduced monocyte numbers, but both latter within the reference range for the species. Albumin concentrations were low in positive cases and remained below the reference range limit for both groups. However, triglycerides exceeded the physiological range for the species in both positive and negative groups. Increased gamma-glutamyltransferase (GGT) activity was observed in positive animals. In conclusion, Crioula Lageana cattle exhibited enzootic instability with a low T. evansi infection prevalence when assessed using PCR and IIFR techniques. Furthermore, the animals did not display clinical, hematological, or biochemical alterations attributable to the presence of hemoparasites. •Polymerase Chain Reaction used to diagnose Trypanosoma evansi.•The prevalence of T. evansi in Crioula Lageana cattle indicates enzootic instability.•Indirect Immunofluorescence Reaction usage on T. evansi diagnosis.•Natural infection by T. evansi doesn't necessarily cause clinical symptoms.