Rat hybridoma antibodies against canine parvovirus

Hybrid cells have been produced by fusion between rat myeloma cells IR983F and spleen cells from a LOU rat immunized with canine enteritis parvovirus. Four stable cell lines were selected each secreting a monoclonal antibody directed against this virus. By indirect immunofluorescence, the antigenic...

Full description

Saved in:
Bibliographic Details
Published inArchives of virology Vol. 71; no. 4; p. 291
Main Authors Burtonboy, G, Bazin, H, Delferriere, N
Format Journal Article
LanguageEnglish
Published Austria 01.01.1982
Subjects
Animals
Antibodies, Monoclonal
Antibodies, Viral - immunology
Antigen-Antibody Reactions
Antigens, Viral - analysis
Epitopes
Neoplasm Transplantation
Parvoviridae - immunology
Rats
Online AccessGet more information

Cover

More Information
Summary:Hybrid cells have been produced by fusion between rat myeloma cells IR983F and spleen cells from a LOU rat immunized with canine enteritis parvovirus. Four stable cell lines were selected each secreting a monoclonal antibody directed against this virus. By indirect immunofluorescence, the antigenic determinants recognized by these antibodies were found to be present not only in canine parvovirus infected cells but also in cells infected by the related parvoviruses of mink enteritis and feline panleukopenia. Two of the monoclonal antibodies inhibit hemagglutination by the canine virus and neutralize its infectivity. Hybrid cells were transplanted into histocompatible animals, and large amounts of the antibodies were obtained.
ISSN:0304-8608
DOI:10.1007/BF01315059