Pneumococcal pneumonia and endotoxemia: An experimental and clinical reappraisal

Background Circulating endotoxins could result from bacterial digestive translocation during sepsis, thus contributing to uncontrolled systemic inflammation, leading in turn to organ dysfunction. We addressed this issue in the setting of severe pneumococcal pneumonia. Methods Endotoxemia was measure...

Full description

Saved in:
Bibliographic Details
Published inEuropean journal of clinical investigation Vol. 54; no. 1; pp. e14077 - n/a
Main Authors Godon, Jeanne, Charles, Pierre‐Emmanuel, Nguyen, Sylvie, Barros, Jean‐Paul Pais, Choubley, Hélène, Jacquier, Marine, Tetu, Jennifer, Quenot, Jean‐Pierre, Luu, Maxime, Binquet, Christine, Masson, David, Piroth, Lionel, Blot, Mathieu, Croisier, Delphine, Gohier, Sandrine, Charles, Carole, Guilloteau, Adrien, Bardou, Marc, Guezala, Ines Ben, Gauthier, Thomas, Bergas, Victoria
Format Journal Article
LanguageEnglish
Published England Blackwell Publishing Ltd 01.01.2024
Subjects
Animals
Chromatography, Liquid
Endotoxemia
Endotoxins
Humans
immune response
Inflammation
Lipid A
Lipids
lipopolysaccharide
Lipopolysaccharides
Mass spectrometry
Mass spectroscopy
Pneumonia
Pneumonia, Pneumococcal - diagnosis
Rabbits
Sepsis
Streptococcus pneumoniae
Tandem Mass Spectrometry
Translocation
Ventilation
pneumonia
lipopolysaccharide
immune response
endotoxemia
Streptococcus pneumoniae
Online AccessGet full text

Cover

More Information
Summary:Background Circulating endotoxins could result from bacterial digestive translocation during sepsis, thus contributing to uncontrolled systemic inflammation, leading in turn to organ dysfunction. We addressed this issue in the setting of severe pneumococcal pneumonia. Methods Endotoxemia was measured in a clinically relevant rabbit model of ventilated pneumococcal pneumonia and in 110 patients with bacteraemic pneumonia, using a patented mass spectrometry (LC–MS/MS) method for detection of 3‐OH fatty acids (C10, C12, C14, C16 and C18), which are molecules bound to the lipid A motif of LPS. Results Whereas higher levels of systemic inflammation and organ dysfunctions were found, there was no significant difference in lipopolysaccharide concentrations when infected rabbits were compared to non‐infected ones, or when patients were compared to healthy volunteers. Conclusions Seemingly, endotoxins do not drive the overwhelming inflammation associated with severe forms of pneumococcal pneumonia.
Bibliography:SourceType-Other Sources-1
content type line 63
ObjectType-Correspondence-1
ISSN:0014-2972
1365-2362
DOI:10.1111/eci.14077