PCR - RFLP patterns for the differentiation of the Fusarium species in virtue of ITS rDNA

• Published in: Current Medical Mycology Vol. 1; no. 1; pp. 4 - 11
• Main Authors: Kachuei, R, Yadegari, M H, Safaie, N, Ghiasian, A, Noorbakhsh, F, Piranfar, V, Rezaie, S
• Format: Journal Article
• Language: English
• Published: Iran Iranian Society of Medical Mycology 01.03.2015; Mazandaran University of Medical Sciences
• Subjects: Fusarium; ITS rDNA; Original; PCR-RFLP; ITS rDNA; PCR-RFLP; Fusarium spp
• ISSN: 2423-3439; 2423-3420; 3420-2423
• DOI: 10.18869/acadpub.cmm.1.1.4

The species are among the most important fungi in the medical, veterinary and agricultural fields. In the present study, 172 strains of these fungi have been analyzed. The high molecular weight DNAs were extracted from 23 reference strains as well as from 149 isolated species. Using the designed nucleotide primers from rDNA of species, PCR analysis was performed for the amplification of ITS regions. Afterwards, the location of the effective endonuclease enzymes has been evaluated within approximately 930 bp of rDNA sequence. Through the selected enzymes including; I, I, I and I, the mentioned species have been divided into 33 groups. The first three enzymes were able to classify species into 23 groups of which 19 groups included one member, one group included two members and three groups included three members of the species. This study also revealed the possibility in the identification of , complex, , , and species by one unique enzyme. In addition, our study indicated the ability of the differentiation of from . As Compared to previous studies with more endonuclease enzymes and with limited in identifications, the ITS-RFLP patterns reported here an attempted to evaluate most of the species successfully.