Specific antibody responses to Qβ‐displayed Plasmodium falciparum‐derived UB05 and MSP3 proteins in mother–neonate couples

• Published in: Scandinavian journal of immunology Vol. 100; no. 5; pp. e13404 - n/a
• Main Authors: Lissom, Abel, Megnekou, Rosette, Tchouangueu, Thibau Flaurant, Ngu, Loveline, Djontu, Jean Claude, Ouambo, Herve Fotso, Sanders, Carrie, Tchadji, Jules Colince, Sake, Carole Stephanie, Tchuandom, Salomon Bonsi, Bawage, Swapnil, Okoli, Arinze Stanley, Park, Chae Gyu, Waffo, Alain Bopda, Godwin, Nchinda Wapimewah
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.11.2024
• Subjects: Adult; Antibodies; Antibodies, Protozoan - blood; Antibodies, Protozoan - immunology; Antibody Formation - immunology; antibody responses; Antigens; Antigens, Protozoan - immunology; Blood parasites; Cord blood; Enzyme-linked immunosorbent assay; Female; Humans; Immunoglobulin G; Immunoglobulin G - blood; Immunoglobulin G - immunology; Infant, Newborn; Malaria; Malaria Vaccines - immunology; Malaria, Falciparum - immunology; Malaria, Falciparum - parasitology; Male; Mothers; mother–neonate couple; MSP3; natural immunity; Neonates; Newborn babies; Peripheral blood; Plasma levels; Plasmodium falciparum; Plasmodium falciparum - immunology; Pregnancy; Protozoan Proteins - immunology; UB05; Young Adult; mother–neonate couple; UB05; natural immunity; MSP3; antibody responses
• ISSN: 0300-9475; 1365-3083; 1365-3083
• DOI: 10.1111/sji.13404

Malaria blood‐stage parasite is a critical pathogenic stage responsible for serious adverse outcomes in pregnant women and their neonates. Immunoglobulin G (IgG) antibody responses specific to various asexual blood‐stage antigens were well reported in non‐pregnant individuals. However, little is still known during placental malaria. To assess the antibody responses specific to Plasmodium falciparum‐derived MSP3 and UB05 malaria vaccine candidates in mother‐neonate couples, mother's peripheral blood and neonate's cord blood samples were collected at delivery. After malaria diagnostic, plasma levels of IgG and IgG subclass responses specific to UB05, MSP3 and UB05‐MSP3 were determined using ELISA. As outcomes, both mothers and neonates had significantly higher IgG responses to UB05 and UB05‐MSP3 compared to anti‐MSP3 IgG (p < 0.05), irrespective of malaria status. Significant negative correlations were observed between IgG levels specific to the three antigens and parasitaemia (p < 0.01). Anti‐UB05 and anti‐UB05‐MSP3 IgG levels in neonates showed a significant positive correlation with the corresponding mothers' antibodies (rs = 0.25 with p = 0.04; rs = 0.31 with p = 0.01, respectively). UB05MSP3‐specific IgG3 and IgG1 subclass responses were significantly higher than the IgG4 subclass (p < 0.01). The neonates IgG1 and IgG3 levels positively correlated with the corresponding antibody subclasses of mothers. These findings suggest an association between UB05 and UB05‐MSP3‐specific antibody responses and malaria control during pregnancy. Maternal–foetal transfer of MSP3 and UB05‐specific IgG occurs during pregnancy, suggesting the interest in the future malaria vaccination strategies in pregnant women to generate early protective immunity in baby against malaria. The present study showed an association between anti‐UB05 and anti‐UB05‐MSP3 antibody responses and malaria control during pregnancy. Maternal–foetal transfer of MSP3 and UB05‐specific IgG occurs during pregnancy, suggesting the interest in the future malaria vaccination strategies in pregnant women to generate early protective immunity in baby.