Molecular dynamics simulations corroborate recombinant expression studies carried out on three αIIb β‐propeller mutations reported in Indian Glanzmann thrombasthenia patients

• Published in: Journal of cellular biochemistry Vol. 124; no. 7; pp. 989 - 1001
• Main Authors: Chandrasekaran, Finola Priyadharshini, Vishal, Adarsh, Arora, Udita, Kumar S., Udhaya, George C., Priya Doss, Nelson, Everette Jacob Remington
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.07.2023
• Subjects: Dynamic stability; Dynamic structural analysis; Evolutionary conservation; Glanzmann thrombasthenia; Humans; Hydrogen bonding; Hydrogen bonds; Integrin alpha2 - genetics; Integrin alpha2 - metabolism; Integrin beta3 - genetics; Intracellular; missense mutations; Molecular dynamics; Molecular Dynamics Simulation; molecular dynamics simulations; Mutants; Mutation; Phenotypes; Platelet Glycoprotein GPIIb-IIIa Complex - genetics; Platelet Glycoprotein GPIIb-IIIa Complex - metabolism; Protein structure; Protein Structure, Secondary; Protein transport; Secondary structure; Simulation; Stability analysis; Thrombasthenia - genetics; Thrombasthenia - metabolism; αIIbβ3; β‐propeller; molecular dynamics simulations; αIIbβ3; Glanzmann thrombasthenia; β-propeller; missense mutations
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.30423

Mutations in the αIIb β‐propeller domain have long been known to disrupt heterodimerization and intracellular trafficking of αIIbβ3 complexes leading to diminished surface expression and/or function, resulting in Glanzmann thrombasthenia. Our previous study on three β‐propeller mutations, namely G128S, S287L, and G357S, showed variable defects in protein transport correlated with the patient's clinical phenotypes. Pulse‐chase experiments revealed differences in αIIbβ3 complex maturation among the three mutations. Hence, the current study aims to correlate conformational changes caused by each one of them. Evolutionary conservation analysis, stability analysis, and molecular dynamics simulations of the three mutant structures were carried out. Stability analysis revealed that, while G128S and G357S mutations destabilized the β‐propeller structure, S287L retained the stability. Wild‐type and mutant β‐propeller structures, when subjected to molecular dynamics simulations, confirmed that G128S and G357S were both destabilizing in nature when compared with the wild‐type and S287L based on several parameters studied, like RMSD, RMSF, Rg, FEL, PCA, secondary structure, and hydrogen bonds. In our previous study, we demonstrated that mutant S287L αIIbβ3 complexes were more stable than the wild‐type αIIbβ3 complexes, as evidenced in pulse‐chase experiments. These findings corroborate variable intracellular fates of mutant αIIbβ3 complexes as a result of these β‐propeller mutations.