WntA and Wnt4 during the regeneration of internal organs in the holothurian Eupentacta fraudatrix

• Published in: Genesis (New York, N.Y. : 2000) Vol. 62; no. 1; pp. e23562 - n/a
• Main Author: Girich, A. S.
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.02.2024; Wiley Subscription Services, Inc
• Subjects: Body wall; Connective tissues; Epithelium; Eupentacta fraudatrix; Gene expression; Genes; holothurians; migration; Muscles; Nerves; Proteins; Regeneration; signaling; Wnt; Wnt protein; migration; holothurians; signaling; Wnt; regeneration
• ISSN: 1526-954X; 1526-968X; 1526-968X
• DOI: 10.1002/dvg.23562

Summary Background Over the past few years, it has been established that wnt genes are involved in the regenerative processes of holothurians. The wnt4 gene was identified as one of the most active genes in Eupentacta fraudatrix regeneration using differential gene expression analysis and qPCR of individual genes. Also, the wntA gene was found in holothurians, which is present only in invertebrates and can perform unique functions. Results In this regard, both these genes and proteins were studied in this work. During regeneration, the Wnt4 protein is found in the cells of the coelomic and ambulacral epithelium, retractor muscles, and radial nerves. Single cells with this protein are also found in the connective tissue of the developing aquapharyngeal bulb and in the hypoderm of the body wall. Cells with WntA are found exclusively in the hypoderm of the body wall. Conclusion We assume that both genes are involved in regeneration, but Wnt4 coordinates the formation of the epithelial tissue structure, while WntA maintains the state of the intercellular substance of the body wall.