In vitro and in vivo investigation of Argiope bruennichi spider silk‐based novel biomaterial for medical use

• Published in: Biopolymers Vol. 115; no. 3; pp. e23572 - n/a
• Main Authors: Karahisar Turan, Seçil, Kılıç Süloğlu, Aysun, İde, Semra, Türkeş, Tuncay, Barlas, Nurhayat
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.05.2024; Wiley Subscription Services, Inc
• Subjects: Allergic reactions; Allergies; Aloe vera; Animals; Argiope; Argiope bruennichi; Biocompatibility; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Biomaterials; Biomedical materials; Cell Line; Cytotoxicity; Hematology; histology; In vivo methods and tests; Male; Materials Testing; Mechanical tests; Mice; Rats; Rats, Wistar; SAXS analysis; Scattering, Small Angle; Silk; Silk - chemistry; Skin - drug effects; Small angle X ray scattering; spider silk; Spiders - chemistry; Surgical equipment; Sutures; Wound healing; Wound Healing - drug effects; X-Ray Diffraction; Argiope bruennichi; SAXS analysis; wound healing; Aloe vera; spider silk; histology
• ISSN: 0006-3525; 1097-0282
• DOI: 10.1002/bip.23572

As a natural and biocompatible material with high strength and flexibility, spider silk is frequently used in biomedical studies. In this study, the availability of Argiope bruennichi spider silk as a surgical suture material was investigated. The effects of spider silk‐based and commercial sutures, with and without Aloe vera coating, on wound healing were evaluated by a rat dorsal skin flap model, postoperatively (7th and 14th days). Biochemical, hematological, histological, immunohistochemical, small angle x‐ray scattering (SAXS) analyses and mechanical tests were performed. A. bruennichi silk did not show any cytotoxic effect on the L929 cell line according to MTT and LDH assays, in vitro. The silk materials did not cause any allergic reaction, infection, or systemic effect in rats according to hematological and biochemical analyses. A. bruennichi spider silk group showed a similar healing response to commercial sutures. SAXS analysis showed that the 14th‐day applications of A. bruennichi spider silk and A. vera coated commercial suture groups have comparable structural results with control group. In conclusion, A. bruennichi spider silk is biocompatible in line with the parameters examined and shows a healing response similar to the commercial sutures commonly used in the skin.