DNA barcodes for the pipefish genus Corythoichthys (Actinopterygii: Syngnathiformes) from the Indian Ocean provide insights into cryptic diversity

• Published in: Journal of fish biology Vol. 102; no. 3; pp. 680 - 688
• Main Authors: Shalu, Kannan, Thomas, Liju, Ramvilas, Ghosh, Shabeena, Kadapurathillam S., Philip, Siby, Sureshkumar, Sivanpillai, Raghavan, Rajeev, Ranjeet, Kutty
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.03.2023; Wiley Subscription Services, Inc
• Subjects: Animals; Biodiversity; Classification - methods; conservation; Corythoichthys; cryptic species; Deoxyribonucleic acid; DNA; DNA barcoding; DNA Barcoding, Taxonomic; Gene sequencing; Genetic diversity; Genetic variation; Geographical distribution; Indian Ocean; Marine fishes; Phylogenetics; Phylogeny; Smegmamorpha - classification; Smegmamorpha - genetics; Species; Species diversity; Species Specificity; syngnathidae; Systematics; Taxonomy; Indian Ocean; conservation; syngnathidae; taxonomy; cryptic species
• ISSN: 0022-1112; 1095-8649
• DOI: 10.1111/jfb.15300

The syngnathiform genus Corythoichthys comprises a group of taxonomically complex, tail‐brooding (Syngnathinae) pipefishes widely distributed in the Indo‐Pacific region. Due to the presence of overlapping interspecific morphological characters, reliable taxonomic information on Corythoichthys is still lacking. Using 52 CO1 sequences, including seven newly generated, a phylogenetic analysis was carried out to understand the genetic diversity, distribution and ‘species groups’ within the genus Corythoichthys. Species delimitation using Automatic Barcode Gap Discovery (ABGD) analysis confirmed the presence of 13 species which include ‘species‐complexes’ previously considered as a single taxon. Our results revealed the presence of three species groups, ‘C. amplexus’, ‘C. conspicillatus’ and ‘C. haematopterus’ and four unidentified/undescribed species in the wider Indo‐Pacific realm. Interestingly, 60 sequences and a mitogenome identified as Corythoichthys in GenBank are misidentified at the genus level. Based on our findings, we suggest that the taxonomy and systematics of Corythoichthys need to be re‐examined and validated using integrative methods, and care should be taken while selecting specimens for genetic studies.