Effect of Spleen Lymphocytes on the Splenomegaly in Hepatocellular Carcinoma-bearing Mice

• Published in: Biomedical and environmental sciences Vol. 27; no. 1; pp. 17 - 26
• Main Authors: FANG, Jing Jing, ZHU, Zhen Yuan, DONG, Hui, ZHENG, Guo Qiang, TENG, An Guo, LIU, An Jun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 2014; Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China
• Subjects: Animals; Carcinoma, Hepatocellular - complications; Cell Cycle; Female; Hepatocellular carcinoma; IL-2; Liver Neoplasms - complications; Lymphocytes; Lymphocytes - physiology; Mice; Mice, Inbred ICR; Neoplasms, Experimental - therapy; S100-A9; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spleen - cytology; Spleen - pathology; Splenomegaly; Splenomegaly - etiology; Splenomegaly - therapy; Thymus Gland; T淋巴细胞; β-actin; 二维凝胶电泳; 淋巴细胞亚群; 肝癌; 脾淋巴细胞; 脾肿大; 荷瘤小鼠; Splenomegaly; Hepatocellular carcinoma; β-actin; S100-A9; Lymphocytes
• ISSN: 0895-3988; 2214-0190
• DOI: 10.3967/bes2014.012

Objective To study the effect of spleen lymphocytes on the splenomegaly by hepatocellular carcinoma-bearing mouse model. Methods Cell counts, cell cycle distribution, the percentage of lymphocytes subsets and the levels of iL-2 were measured, and two-dimensional gel electrophoresis （2-DE） was used to investigate the relationship between spleen lymphocytes and splenomegaly in hepatocellular carcinoma-bearing mice. Results Compared with the normal group, the thymus was obviously atrophied and the spleen was significantly enlarged in the tumor-bearing group. Correlation study showed that the number of whoJe spleen cells was positively correlated with the splenic index. The cell diameter and cell-cycle phase distribution of splenocytes in the tumor-bearing group showed no significant difference compared to the normal group. The percentage of CD3＋ T lymphocytes and CD8＋ T lymphocytes in spleen and peripheral blood of tumor-bearing mice were substantially higher than that in the normal mice. Meanwhile, the IL-2 level was also higher in the tumor-bearing group than in the normal group. Furthermore, two dysregulated protein, β-actin and .S100-A9 were identified in spleen lymphocytes from H22-bearing mice, which were closely related to cellular motility. Conclusion It is suggested that dysregulated β-actin and S100-A9 can result in recirculating T lymphocytes trapped in the spleen, which may explain the underlying cause of splenomegaly in H22-bearing mice.