Mesenchymal stromal cells synchronize the rhythm of protein synthesis under the effect of an exogenous signal

• Published in: Russian journal of developmental biology Vol. 43; no. 3; pp. 196 - 198
• Main Authors: Brodsky, V. Ya, Vasil’ev, A. V, Terskikh, V. V, Zvezdina, N. D, Fateeva, V. I, Mal’chenko, L. A, Kiseleva, E. V, Bueverova, E. I
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.05.2012; SP MAIK Nauka/Interperiodica
• Subjects: adipose tissue; Animal Anatomy; Biomedical and Life Sciences; bone marrow; calcium; chelating agents; culture media; Developmental Biology; hepatocytes; Histology; humans; Life Sciences; melatonin; Morphology; phosphorylation; protein synthesis; proteins; rats; Short Communication; stromal cells; cell interactions; protein synthesis rhythm; circahoralian rhythms; hepatocytes; mesenchymal stromal cells
• ISSN: 1062-3604; 1608-3326
• DOI: 10.1134/S1062360412030022

A comparative study was performed of dense 5-hour cultures of rat hepatocytes and equal-density cultures of mesenchymal stromal cells (MSC) isolated from human adipose tissue of rat bone marrow. The cells were grown on collagen-coated class slides in serum-free medium. Unlike in hepatocytes, no rhythm of protein synthesis was initially revealed in MSC, but such a rhythm manifested itself when the culture medium was supplemented with melatonin (2 nM, 5 min). The results of experiments with cytoplasmic calcium chelator BAPTA-AM and protein kinase inhibitor H7 indicate that the mechanism of protein synthesis synchronization in MSC consists in calcium-dependent phosphorylation of cell proteins.