Mesenchymal stromal cells synchronize the rhythm of protein synthesis under the effect of an exogenous signal
A comparative study was performed of dense 5-hour cultures of rat hepatocytes and equal-density cultures of mesenchymal stromal cells (MSC) isolated from human adipose tissue of rat bone marrow. The cells were grown on collagen-coated class slides in serum-free medium. Unlike in hepatocytes, no rhyt...
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Published in | Russian journal of developmental biology Vol. 43; no. 3; pp. 196 - 198 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer-Verlag
01.05.2012
SP MAIK Nauka/Interperiodica |
Subjects | |
Online Access | Get full text |
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Summary: | A comparative study was performed of dense 5-hour cultures of rat hepatocytes and equal-density cultures of mesenchymal stromal cells (MSC) isolated from human adipose tissue of rat bone marrow. The cells were grown on collagen-coated class slides in serum-free medium. Unlike in hepatocytes, no rhythm of protein synthesis was initially revealed in MSC, but such a rhythm manifested itself when the culture medium was supplemented with melatonin (2 nM, 5 min). The results of experiments with cytoplasmic calcium chelator BAPTA-AM and protein kinase inhibitor H7 indicate that the mechanism of protein synthesis synchronization in MSC consists in calcium-dependent phosphorylation of cell proteins. |
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Bibliography: | http://dx.doi.org/10.1134/S1062360412030022 |
ISSN: | 1062-3604 1608-3326 |
DOI: | 10.1134/S1062360412030022 |