Flux balance analysis of glucose degradation by anaerobic digestion in negative pressure

Flux balance analysis (FBA) has been widely used for detecting internal fluxes of bacteria. In this study, FBA was applied to estimate metabolic phenotype in glucose fermentation under negative pressure. Result revealed that ethanol, propionic acid, and butyric acid were converted to acetate by lowe...

Full description

Saved in:
Bibliographic Details
Published inInternational journal of hydrogen energy Vol. 45; no. 51; pp. 26822 - 26830
Main Authors Huang, Jialong, Hou, Jingyu, Li, Leigang, Wang, Yuanyuan
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 16.10.2020
Subjects
Anaerobic digestion
Flux balance analysis
Gibbs free energy
Hydrogen fermentation
Negative pressure
Gibbs free energy
Flux balance analysis
Negative pressure
Anaerobic digestion
Hydrogen fermentation
Online AccessGet full text

Cover

More Information
Summary:Flux balance analysis (FBA) has been widely used for detecting internal fluxes of bacteria. In this study, FBA was applied to estimate metabolic phenotype in glucose fermentation under negative pressure. Result revealed that ethanol, propionic acid, and butyric acid were converted to acetate by lowering headspace pressure (HP) of fermenter with the conversion rate of 0.091, 0.014, and 0.017 mol-acetate/mol-glucose respectively In addition, these three substances were simultaneously converted into H2 with the conversion rate of 0.258 mol-H2/mol-glucose. A total of 30% acetate and 26% H2 production increase were observed under condition of 0.6 atm HP fermentation. Furthermore, 30% accumulated hydrogen was consumed in the formation of propanol and valeric acid. [Display omitted] •Revealing three H2-producing pathways induced in negative headspace pressure.•A new FBA model containing new pathways was established.•30% HAc and 26% H2 increased yield were observed under 0.6 atm HP condition.
ISSN:0360-3199
1879-3487
DOI:10.1016/j.ijhydene.2020.07.053