Simple analysis of α-amanitin and β-amanitin in human plasma by liquid chromatography-mass spectrometry

• Published in: Forensic toxicology Vol. 28; no. 2; pp. 110 - 114
• Main Authors: Tanahashi, Masakazu, Kaneko, Rina, Hirata, Yukari, Hamajima, Makoto, Arinobu, Tetsuya, Ogawa, Tadashi, Ishii, Akira
• Format: Journal Article
• Language: English
• Published: Japan Springer Japan 01.07.2010
• Subjects: Forensic Medicine; Forensic Science; Medical Law; Medicinal Chemistry; Medicine; Medicine & Public Health; Pharmacology/Toxicology; Short Communication; β-Amanitin; Plasma; Discovery DSC 18 column; LC-MS (-MS); α-Amanitin; Solid-phase extraction
• ISSN: 1860-8965; 1860-8973
• DOI: 10.1007/s11419-010-0098-6

A number of reports are available in the literature that describe liquid chromatography-mass spectrometry (LC-MS) and LC-tandem mass spectrometry (LC-MS-MS) analysis of amanitins, very toxic mushroom toxins, in biological samples. However, the extractive pretreatment methods and LC separation column materials vary remarkably according to the different reports. This communication presents a very simple and suffi ciently sensitive method for LC-MS analysis of amanitins. A plasma sample was diluted with distilled water and buffer solution, and applied to a Discovery DSC 18 column (500 mg packing material), followed by washing with distilled water and elution with methanol. The extract, after evaporation and reconstitution in mobile phase solution, was subjected to LC-MS analysis with a conventional octadecyl LC separation column. The selected ion monitoring of α-amanitin and β-amanitin at m/z 919–921 and m/z 920–922, respectively, gave symmetrical peaks and good separation of both amanitin peaks. Using an external calibration method, linearity, detection limits, recovery rates, and precision were tested; they were all satisfactory. To our knowledge, the present method gives the simplest LC-MS analysis for amanitins among those so far reported. We recommend the method for use in actual forensic and clinical toxicological analysis of amanitins in biological samples.