High efficiency single-cell capture based on microfluidics for single cell analysis

A microfluidic droplet platform based on flow resistance has been designed to generate, capture and analyze a single cell. In this device, a single cell was encapsulated in micro-droplet form and positioned precisely in arrays of sloped channels on a polydimethylsiloxane-glass chip. The droplets wer...

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Published inJournal of micromechanics and microengineering Vol. 29; no. 3; pp. 35004 - 35009
Main Authors Fan, Weihua, Qiao, Miaomiao, Jin, Yan, Zhou, Hongbo, Ge, Yuqing, Jin, Qinghui, Zhao, Jianlong
Format Journal Article
LanguageEnglish
Published IOP Publishing 01.03.2019
Subjects
hydrodynamics
microdroplets
single-cell capture and analysis
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Summary:A microfluidic droplet platform based on flow resistance has been designed to generate, capture and analyze a single cell. In this device, a single cell was encapsulated in micro-droplet form and positioned precisely in arrays of sloped channels on a polydimethylsiloxane-glass chip. The droplets were formed at the 'T-junction' and trapped by the 'main-bypass' construction. The single cell capture efficiency was up to 60%. Besides, the device caused no harm to living cells as the microdroplet formed here provided a relatively stable micro-environment. The device had the advantages of reagent economy, passive driving, and minimal cell damage, making it a powerful tool for single-cell analysis.
Bibliography:JMM-103846.R1
ISSN:0960-1317
1361-6439
DOI:10.1088/1361-6439/aaf9df