Nucleoside phosphocholine amphiphile for in vitro DNA transfection

A new transfection reagent based on nucleoside phosphocholine amphiphile leading to high transfection efficacy and low cytotoxicity is described. TEM, ethidium bromide displacement assays, agarose gel electrophoresis and SAXS studies support the formation of lipoplexes for the transfection of CHO ce...

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Bibliographic Details
Published inMolecular bioSystems Vol. 1; no. 3; p. 260
Main Authors Moreau, Louis, Barthélémy, Philippe, Li, Yougen, Luo, Dan, Prata, Carla A H, Grinstaff, Mark W
Format Journal Article
LanguageEnglish
Published England 01.09.2005
Subjects
Animals
beta-Galactosidase - genetics
beta-Galactosidase - metabolism
CHO Cells
Cricetinae
DNA - chemistry
DNA - genetics
DNA - ultrastructure
Fatty Acids, Monounsaturated
Genes, Reporter
Indicators and Reagents
Microscopy, Electron
Phosphorylcholine
Plasmids
Quaternary Ammonium Compounds
Recombinant Proteins - metabolism
Transfection - methods
Uridine - analogs & derivatives
X-Ray Diffraction
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Summary:A new transfection reagent based on nucleoside phosphocholine amphiphile leading to high transfection efficacy and low cytotoxicity is described. TEM, ethidium bromide displacement assays, agarose gel electrophoresis and SAXS studies support the formation of lipoplexes for the transfection of CHO cells.
ISSN:1742-206X
DOI:10.1039/b503302k