influence of the limk1 gene polymorphism on learning acquisition and memory formation, pCREB distribution and aggregate formation in neuromuscular junctions in Drosophila melanogaster

• Published in: Russian journal of genetics Vol. 51; no. 6; pp. 582 - 590
• Main Authors: Kaminskaya, A. N, Nikitina, E. A, Medvedeva, A. V, Gerasimenko, M. S, Chernikova, D. A, Savvateeva-Popova, E. V
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.06.2015
• Subjects: adults; Animal Genetics and Genomics; Biomedical and Life Sciences; Biomedicine; courtship; Drosophila melanogaster; General Genetics; genes; genetic polymorphism; Human Genetics; imagos; memory; Microbial Genetics and Genomics; mutants; nerve tissue; synapse; Courtship Behavior; Sound Production; Dorsal Nerve; Neuromuscular Junction; Beta Amyloid Precursor Protein
• ISSN: 1022-7954; 1608-3369
• DOI: 10.1134/S1022795415060071

We have shown previously that the polymorphic structure of the limk1 gene in Drosophila leads to changes in LIMK1 content and to defects in courtship behavior, sound production, and learning/memory. The results of the present study of three wild-type strains and mutant agn ᵗˢ³ with altered limk1 structure demonstrate that long-term memory is normal in Canton-S and Oregon-R but is impaired in Berlin and drastically suppressed in agn ᵗˢ³ . This temperature-sensitive mutant carries the S-element from the Tc1/mariner family insertion near the dlimk1 3′-UTR and, compared to Canton-S, has a reverse pCREB distribution in adult neuromuscular junctions (NMJ) of the second dorsal imago nerve before and after learning. Moreover, only agn ᵗˢ³ demonstrates amyloid-like aggregate formation in NMJ. This suggests that this impedes pCREb transport and thereby impairs the formation of short- and long-term memory.