ELISA and HPLC analysis of ochratoxin A in red wines of Croatia

Ochratoxin A (OTA) contaminates wine all round the world, and its consumption may significantly increase human exposure to this toxin. In this study, enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC) for OTA analysis were tested on must and wine samples coll...

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Bibliographic Details
Published inFood control Vol. 20; no. 6; pp. 590 - 592
Main Authors Flajs, D., Domijan, A.-M., Ivić, D., Cvjetković, B., Peraica, M.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.06.2009
Subjects
Aspergillus carbonarius
ELISA
HPLC
Ochratoxin A
Aspergillus carbonarius
Ochratoxin A
HPLC
ELISA
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Summary:Ochratoxin A (OTA) contaminates wine all round the world, and its consumption may significantly increase human exposure to this toxin. In this study, enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC) for OTA analysis were tested on must and wine samples collected in Croatia. The results of ELISA and HPLC analysis of OTA in naturally contaminated red wines correlated well ( r = 0.821), and the correlation was better at higher OTA concentrations. In contrast to HPLC, ELISA could not detect very low OTA concentrations. OTA concentrations in must (range 19–50 ng/l) were higher than in the wines (range 0–21 ng/l). No must samples showed the presence of Aspergillus carbonarius, which is a common OTA-producing mould affecting grapes.
ISSN:0956-7135
1873-7129
DOI:10.1016/j.foodcont.2008.08.021