Inheritable changes in miRNAs expression in HeLa cells after X-ray and mitomycin C treatment

• Published in: Russian journal of genetics Vol. 50; no. 8; pp. 798 - 806
• Main Authors: Tarasov, V. A, Matishov, D. G, Shin, E. F, Boyko, N. V, Timoshkina, N. N, Makhotkin, M. A, Lomonosov, A. M, Kirpiy, A. A
• Format: Journal Article
• Language: English
• Published: Moscow Springer-Verlag 2014; Pleiades Publishing
• Subjects: Animal Genetics and Genomics; Biomedical and Life Sciences; Biomedicine; epigenetics; Human Genetics; humans; inheritance (genetics); Microbial Genetics and Genomics; microRNA; mitomycin; Molecular Genetics; mutagenicity; oncogenes; tumor suppressor genes; X-radiation; miRNA Expression; Intact Control; miRNA Expression Level; Transcriptional Gene Silence; miRNAs Expression
• ISSN: 1022-7954; 1608-3369
• DOI: 10.1134/S1022795414080092

We identified 40 miRNAs with inherited aberrant expression by multiple parallel sequencing of human HeLa cells irradiated with X rays and mitomycin C. Twenty-two miRNAs were repressed and 15 miRNAs were induced after radiation and mytomycin C treatment. The expression of three miRNAs (miR-10b-5p, miR-148a-3p, and miR-340-5p) decreased after X-ray exposure and increased after mitomycin C treatment. The spectrum of aberrantly expressed miRNAs after X-ray and mitomycin C treatment is different, except for three miRNAs (mir-100-5p, miR-99b-5p, miR-501-3p), which showed the inherited decreased expression after both mutagens. It has been ascertained that for five miRNAs (miR-21-3p, miR-182-5p, miR-19b-3p, miR-30a-3p, and miR-30e-3p) with increased inherited expression, the targets are well-described tumor suppressor genes. For 9 miRNAs (miR-99b-5p, miR-148a-3p, miR-365a-3p, miR-193a-3p, miR-100-5p, miR-99a-5p, miR-29b-3p, miR-340-5p, and miR-23b-3p) with reduced inherited expression, the targets are oncogenes. The obtained results provide further support of the idea that induced epigenetic changes in the genome should be considered when assessing the long-term genetic effects of ionizing radiation and chemical compounds.