introduction of the 9α-hydroxy group into androst-4-en-3,17-dione using a new actinobacterium strain

A 9α-hydrolase activity of a new actinobacterium strain identified as Rhodococcus erythropolis based on the analysis of a 16S rRNA gene sequence (1417 nucleotides) towards androst-4-en-3,17-dione (AD) was studied. In the presence of glucose in the medium, this strain completely transformed AD (4-20...

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Published inApplied biochemistry and microbiology Vol. 45; no. 4; pp. 395 - 400
Main Authors Rodina, N. V, Andryushina, V. A, Stytsenko, T. S, Turova, T. P, Baslerov, R. V, Panteleeva, A. N, Voishvillo, N. E
Format Journal Article
LanguageEnglish
Published Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01.07.2009
SP MAIK Nauka/Interperiodica
Subjects
Biochemistry
Biomedical and Life Sciences
Life Sciences
Medical Microbiology
Microbiology
Rhodococcus
Transformation Medium
DMFA
Rhodococcus Erythropolis
Apply Biochemistry
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Summary:A 9α-hydrolase activity of a new actinobacterium strain identified as Rhodococcus erythropolis based on the analysis of a 16S rRNA gene sequence (1417 nucleotides) towards androst-4-en-3,17-dione (AD) was studied. In the presence of glucose in the medium, this strain completely transformed AD (4-20 g/l) into 9α-hydroxy-AD over 20-48 h. This culture was able to grow and perform AD 9α-hydroxylation at a concentration of dimethyl formamide up to 9%. Crystalline 9α-hydroxy-AD was isolated with a yield of over 90%.
Bibliography:http://dx.doi.org/10.1134/S0003683809040085
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683809040085