The Difference of Sensitivity between BXPC-3 and K562 Cells by Treatments with Combination of Indole-3-acetic Acid and Horseradish Peroxidase

• Published in: Journal of Wuhan University of Technology. Materials science edition Vol. 21; no. 4; pp. 95 - 98
• Main Authors: Yali, Ben, Deli, Liu, Dali, Zhu, Derui, Zhu, Qin, Luo
• Format: Journal Article
• Language: English
• Published: Wuhan Springer Nature B.V 01.12.2006; College of Life Science, HuaZhong Normal University, Wuhan 430079, China; Jianghan University, Wuhan 430016, China%College of Life Science, HuaZhong Normal University, Wuhan 430079, China%Jianghan University, Wuhan 430016, China
• Subjects: Apoptosis; BXPC-3; Cell cycle; K562; 工程材料学; 敏感性; 过氧化物酶; indole-3-acetic acids; apoptosis; K562 cell; BXPC-3 cell; horseradish peroxidase
• ISSN: 1000-2413; 1993-0437
• DOI: 10.1007/BF02841214

The difference of sensitivity to indole- 3-acetic acid （ IAA ） combined with horseradish peroxidase （HRP） in K562 and BXPC- 3 cells was investigated. The cell proliferation was determined by MTF assay. The cell cycle and apoptosis of K562 and BXPC-3 cells were examined by a fluorescence flow cytometer （FCM） and terminal deoxynacleotidyl transferase-mediated dUTP nick-end labeling （TUNEL） respectively. The experimental results show that IAA and HRP could inhibit BXPC- 3 cell proliferation greatly compared with K562 cell during the first 48 h . The cell cycle was arrested predominantly at G2/ M phase in K562 and BXPC- 3 cells. The cell apoptosis of K562 and BXPC- 3 was induced by IAA/ HRP. There was a significant difference between the two cell lines since BXPC-3 cells were more sensitive than K562 cells by treatments with combination of IAA and HRP.