Visual detection of melamine in raw milk by label-free silver nanoparticles

A simple, rapid colorimetric method using label-free Ag NPs (silver nanoparticles) as probe for detection of melamine in raw milk was developed in this study. The assay relies on the fact that melamine can induce aggregation of Ag NPs, and thereby results in their yellow-to-red color change. The con...

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Bibliographic Details
Published inFood control Vol. 23; no. 1; pp. 191 - 197
Main Authors Ping, Hong, Zhang, Minwei, Li, Hongkun, Li, Shugui, Chen, Quansi, Sun, Chunyan, Zhang, Tiehua
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.01.2012
Subjects
Melamine
Silver nanoparticles
Visual detection
Melamine
Visual detection
Silver nanoparticles
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Summary:A simple, rapid colorimetric method using label-free Ag NPs (silver nanoparticles) as probe for detection of melamine in raw milk was developed in this study. The assay relies on the fact that melamine can induce aggregation of Ag NPs, and thereby results in their yellow-to-red color change. The concentration of melamine in raw milk can be determined by monitoring with the naked eyes or a UV–Vis spectrophotometer. The detection limit of the present method for melamine is 2.32 μM (3σ). The proposed method is a promising mean for on-site screening of melamine adulterant in raw milk without costly instruments. ► The Ag NPs-based visual assay was established to detect melamine in raw milk. ► Mechanism was discussed by TEM, spectral analysis and design of control experiments. ► The detection results of melamine in raw milk can be observed by naked eyes and absorption spectra. ► The assay possesses the merits of rapidness, cheapness and simple pretreatment.
ISSN:0956-7135
1873-7129
DOI:10.1016/j.foodcont.2011.07.009