Processes hindering activation of lipid peroxidation in cold-tolerant plants under hypothermia

• Published in: Russian journal of plant physiology Vol. 58; no. 6; pp. 1020 - 1026
• Main Authors: Sin’kevich, M. S, Naraikina, N. V, Trunova, T. I
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.11.2011; SP MAIK Nauka/Interperiodica
• Subjects: antioxidant activity; Biomedical and Life Sciences; catalase; cultivars; hydrogen peroxide; hypothermia; Life Sciences; lipid peroxidation; peroxidase; Plant Physiology; Plant Sciences; potatoes; Research Papers; Solanum tuberosum; superoxide anion; superoxide dismutase; peroxidation of lipids; peroxidase; superoxide dismutase; ROS; low-temperature tolerance and hardening; oxidative stress; catalase
• ISSN: 1021-4437; 1608-3407
• DOI: 10.1134/S1021443711050232

The reasons why the rate of lipid peroxidation (POL) associated with a long-term action of low above-zero temperature (5°C, 6 days) on 6-week-old plants of two potato (Solanum tuberosum L.) cultivars (cold-tolerant cv. Desnitsa and less tolerant cv. Desiree) did not rise were investigated. Upon a long-term action of low hardening temperatures on the plants of both cultivars, there was an equilibrium between the rate of generation of superoxide anion (O 2 ·− and activity of superoxide dismutase (SOD), which inactivated it with the formation of H2O2. Among the enzymes breaking up hydrogen peroxide, the highest activity was observed for guaiacol peroxidases, which was an order of magnitude greater than the activity of catalase. In potato cultivars, POL processes were not considerably activated; however, activities of antioxidant enzymes (SOD, catalase, and guaiacol peroxidases) in cold-tolerant cv. Desnitsa and less tolerant cv. Desiree differed. It was concluded that, upon a long-term action of hardening temperatures, cold-tolerant plants could sup-press POL processes. Moreover, a test for tolerance to damaging temperature (−3°C, 18 h) showed that detected preservation of the prooxidant/antioxidant equilibrium not only maintained vital activities at low above-zero temperatures but also elevated tolerance to short-term frosts, with this adaptability being cultivar-specific.