Inhibitor degradation by Rhodosporidium toruloides NRRL 1588 using undetoxified wood hydrolysate as a culture media

• Published in: Biomass & bioenergy Vol. 160; p. 106419
• Main Authors: Osorio-González, Carlos S., Saini, Rahul, Hegde, Krishnamoorthy, Brar, Satinder Kaur, Lefebvre, Alain, Avalos-Ramírez, Antonio
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.05.2022
• Subjects: Furans; Inhibitory compounds; Organic acids; Phenolic compounds; Rhodosporidium toruloides NRRL 1588; Wood hydrolysate; Wood hydrolysate; Rhodosporidium toruloides NRRL 1588; Inhibitory compounds; Furans; Phenolic compounds; Organic acids
• ISSN: 0961-9534; 1873-2909
• DOI: 10.1016/j.biombioe.2022.106419

Lignocellulosic biomass has been identified as a renewable and sustainable feedstock to produce liquid hydrolysates as a suitable substrate to produce a variety of compounds through biochemical processes. Nevertheless, the main challenge to using this substrate is the hydrolysis needed to release fermentable sugars. This pretreatment leads to the production of microbial toxic compounds such as furans, phenols and organic acids. In this work, an oleaginous yeast, R. toruloides NRRL 1588, was used to study its ability to degrade inhibitors in C5 and C6 wood hydrolysates obtained from forestry residues. The study showed that R. toruloides NRRL 1588 can grow, accumulate lipids, and degrade up to 8.01 mgL−1 h−1 of furfural, 5.63 mgL−1 h−1 of 5-hydroxy methyl furfural, 1.70 mgL−1 h−1 of levulinic acid, 1.15 mgL−1 h−1 of syringaldehyde, 0.67 mgL−1 h−1 of vanillin, and 1.03 mgL−1 h−1 of vanillic acid. This work confirms the robustness of R. toruloides NRRL 1588 when grows in wood hydrolysates containing inhibitory compounds. [Display omitted] •Degradation of furfural, 5-HMF, vanillin, syringaldehyde, vanillic and levulinic acid.•Tolerance of R. toruloides to microbial inhibitory compounds present on wood hydrolysates.•R. toruloides accumulate lipids in presence of microbial inhibitory compounds.•Wood hydrolysates are a potential and renewable microbial culture media.