Structural changes in layers of lipid mixtures at low surface tensions

• Published in: Chemistry and physics of lipids Vol. 258; p. 105365
• Main Authors: Bykov, A G, Panaeva, M A, Milyaeva, O Y, Michailov, A V, Rafikova, A R, Guzman, E, Rubio, R, Miller, R, Noskov, B A
• Format: Journal Article
• Language: English
• Published: Ireland 01.01.2024
• Subjects: 1,2-Dipalmitoylphosphatidylcholine - chemistry; Lipids - chemistry; Lung - chemistry; Microscopy; Surface Properties; Surface Tension; Water - chemistry; Monolayer collapse; Surface elasticity; DPPC; Ellipsometry; Pulmonary lipids
• ISSN: 0009-3084; 1873-2941
• DOI: 10.1016/j.chemphyslip.2023.105365

Layers of pulmonary lipids on an aqueous substrate at non-equilibrium conditions can decrease the surface tension of water to quite low values. This is connected with different relaxation processes occurring at the interface and the associated changes in the surface layer structure. Results of measurements by the combination of methods like surface rheology, ellipsometry, Brewster angle microscopy, and IRRAS for spread layers of lipid mixtures open a possibility to specify the dynamics of structural changes at conditions close to the physiological state. At sufficiently low surface tension values (below 5 mN/m) significant changes in the ellipsometric signal were observed for pure DPPC layers, which can be related to a transition from 2D to 3D structures caused by the layer folding. The addition of other lipids can accelerate the relaxation processes connected with squeezing-out of molecules or multilayer stacks formation hampering thereby a decrease of surface tension down to low values corresponding to the folding of the monolayer.