Sequential G-banding FISH on human sperm chromosomes

A method that allows the performance of double-colour chromosome painting (FISH) on previously G-banded human sperm metaphases has been developed. Sperm chromosomes were obtained by using the fusion technique between zona-free hamster oocytes and human spermatozoa. Single- and double-colour chromoso...

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Bibliographic Details
Published inChromosome research Vol. 5; no. 7; pp. 457 - 461
Main Authors Colls, P, Templado, C, Martínez-Pasarell, O, Darroudi, F, Natarajan, A T
Format Journal Article
LanguageEnglish
Published Netherlands Springer Nature B.V 01.11.1997
Subjects
Adult
Animals
Chromosome Banding
Chromosomes
Chromosomes, Human, Pair 21 - chemistry
Chromosomes, Human, Pair 21 - genetics
Cricetinae
Fluorescein-5-isothiocyanate
Fluorescent Dyes
Humans
In Situ Hybridization, Fluorescence
Indoles
Male
Metaphase - genetics
Rhodamines
Spermatozoa - chemistry
Spermatozoa - cytology
Staining and Labeling
X Chromosome - chemistry
X Chromosome - genetics
Y Chromosome - chemistry
Y Chromosome - genetics
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Summary:A method that allows the performance of double-colour chromosome painting (FISH) on previously G-banded human sperm metaphases has been developed. Sperm chromosomes were obtained by using the fusion technique between zona-free hamster oocytes and human spermatozoa. Single- and double-colour chromosome painting was performed using DNA libraries specific for chromosomes X, Y and 21 on either unstained or G-banded preparations. The hybridization efficiency was very high (98%). The sequential staining technique is very useful for analyses of structural (stable) and numerical chromosome aberrations in human sperm and thus can increase the efficiency of the human sperm-hamster oocytes fusion system to assess the risk to human germ cells as a result of endogenous and exogenous factors.
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ISSN:0967-3849
1573-6849
DOI:10.1023/A:1018464929628