Development of a novel nano-biomaterial for biomedical applications

• Published in: Materials research express Vol. 5; no. 12; pp. 125014 - 125030
• Main Authors: Machado, Vagner de Oliveira, Andrade, Ângela Leão, Simon, Alice, Rodríguez-Fernández, Daniel Ernesto, Fabris, José Domingos, Domingues, Rosana Zacarias, da Silva, Raphael Ferreira, Silva, Thamiris Caroline Esteves, Peixoto, Thainá Lopes, dos Santos, Cláudio Teodoro, Motta, Adriana Cristina, Duek, Eliana Aparecida de Rezende, Silva, Messias Borges, Gomes, Alaelson Vieira, Cabral, Lúcio Mendes, do Carmo, Flávia Almada, Elias, Carlos Nelson
• Format: Journal Article
• Language: English
• Published: IOP Publishing 01.12.2018
• Subjects: box-behnken; finasteride; nanoparticle; nanoprecipitation; PEO-PPO-PEO; PLDLA-co-TMC
• ISSN: 2053-1591; 2053-1591
• DOI: 10.1088/2053-1591/aae11b

The technological development of biodegradable nanoparticles for drug delivery in medical diagnostics and therapies is addressing increasing efforts to make safer, affordable and more bio-efficient polymeric materials. The preparation via the solvent displacement method of a novel polymeric nanomaterial with new spherical and narrow sizes dispersion is described. It was achieved by choosing the best amount of PLDLA-co-TMC, PEO-PPO-PEO and an adequate acetone:methanol volume ratio, using the factorial design 23 and Box-Behnken experiment design. This new material was loaded with finasteride, the chosen drug model, and its delivery dynamic was determined by the HPLC method. Results revealed that an optimal formulation of PLDLA-co-TMC nanoparticles was found to be composed of 20 mg PLDLA-co-TMC; 37.5 mg PEO-PPO-PEO and a volume acetone:methanol ratio 2.2:0.3. The formulation of this novel biomaterial showed a high encapsulation efficiency of finasteride, controlled release profile along 24 h, without burst effect in the first 4 h, nanometric sizes, spherical shapes, close polydispersity, negative surface charge, unimodal particle size distribution and relatively high thermal stability of the polymer matrix PEO-PPO-PEO/PLDLA-co-TMC. These results support the fabrication of technologically advanced nanoparticles based on high molecular weight PLDLA-co-TMC and their use in the nano-controlled drug release in biomedical applications.