Effect of genotype and medium culture content on microspore-derived embryo formation in Chinese cabbage (Brassica rapa ssp. chinensis) cv. Lastochka

The influence of different factors on microspore embryogenesis in Chinese cabbage (B. rapa ssp. chinensis) was studied. A genotype dependence for embryo formation was observed. The majority of embryos and plants were obtained from microspores isolated from flower buds (2–2.9 mm in length) and cultur...

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Published inBiology bulletin of the Russian Academy of Sciences Vol. 42; no. 4; pp. 302 - 309
Main Authors Shumilina, D. V, Shmykova, N. A, Bondareva, L. L, Suprunova, T. P
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 01.07.2015
Subjects
activated carbon
activated charcoal
benzylaminopurine
Biochemistry
Biomedical and Life Sciences
Brassica rapa subsp. chinensis
buds
Cell Biology
Chinese cabbage
culture media
doubled haploids
Ecology
embryo (plant)
embryogenesis
Genetics
genotype
Life Sciences
microspores
sucrose
Zoology
Microspore Culture
Double Haploid
Biology Bulletin
Activate Charcoal
Chinese Cabbage
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Summary:The influence of different factors on microspore embryogenesis in Chinese cabbage (B. rapa ssp. chinensis) was studied. A genotype dependence for embryo formation was observed. The majority of embryos and plants were obtained from microspores isolated from flower buds (2–2.9 mm in length) and cultured in the NLN liquid medium with 13% sucrose (w/v) supplemented with 24-epibrassinolide and 1% activated charcoal. Embryos cultured on the 1/2 Murashige-Skoog culture medium with 2% sucrose (w/v), 0.1 mg/L benzylaminopurine, and 3 g/L Phytagel stimulated the formation of secondary embryos that resulted in development of large number of doubled haploid plants.
Bibliography:http://dx.doi.org/10.1134/S1062359015040135
ISSN:1062-3590
1608-3059
DOI:10.1134/S1062359015040135