Characterization of two novel LPS-binding sites in leukocyte integrin βA domain

• Published in: The FASEB journal Vol. 21; no. 12; pp. 3231 - 3239
• Main Authors: Wong, Kwong-Fai, Luk, John M, Cheng, R. Holland, Klickstein, Lloyd B, Fan, Sheung-Tat
• Format: Journal Article
• Language: English
• Published: The Federation of American Societies for Experimental Biology 01.10.2007
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fj.06-7579com

Lipopolysaccharide (LPS), a bacterial endotoxin, triggers deleterious systemic inflammatory responses when released into blood circulation, causing organ dysfunction and death. In response to LPS stimulation, CD14 and toll-like receptor (TLR)-4 elicit inflammatory signaling cascades. Although leukocyte integrins (CD11b/CD18 and CD11c/CD18) were reported to bind LPS and induce NF-κB translocation, the evidence on such epitope location remains elusive. The present study aims to delineate the LPS-binding sites on the integrin CD18 antigen and to design peptide(s) as potential prophylactic and/or therapeutic agents to modulate LPS effects in activated Jurkat cells. Epitope mapping analysis using a series of CD18 truncated variants revealed two putative LPS-binding sites within the βA region (216-248 and 266-318 a.a.), which were further confirmed by point mutation studies. Inhibition assay demonstrated that the CD18-βA₂₆₆₋₃₁₈ peptide could block LPS binding in a dose-dependent manner. Our data also indicated that treatment with the CD18-peptide modulated TNF-α mRNA transcription via the NF-κB signaling pathway in LPS-activated Jurkat cells. In conclusion, we have identified two novel LPS-binding sites located at the CD18 βA domain of leukocyte integrin, and the integrin peptide βA₂₆₆₋₃₁₈ is shown to inhibit LPS binding and subsequent inflammatory events, having therapeutic implications to cure Gram-negative endotoxemia.-- Wong, K-F, Luk, J. M., Cheng, R. H., Klickstein, L. B., and Fan, S-T. Characterization of two novel LPS-binding sites in leukocyte integrin βA domain.