Insilco prediction of the role of the FriZZled5 gene in colorectal cancer

• Published in: Cancer treatment and research communications Vol. 36; p. 100751
• Main Authors: Hosseini-Abgir, Alireza, Naghizadeh, Mohammad mehdi, Igder, Somayeh, Miladpour, Behnoosh
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 2023; Elsevier
• Subjects: Bioinformatics; CRC; FZD5; IBD; microRNA; Systems biology; microRNA; IBD; FZD5; CRC; Systems biology; Bioinformatics
• ISSN: 2468-2942; 2468-2942
• DOI: 10.1016/j.ctarc.2023.100751

•FZD-5 gene expression reduces in both IBD and CRC groups.•Increased expression of miR-191 may lead to CRC and IBD by inhibiting of FZD5 gene.•Increased expression of miR-885-5p may related to the inhibition of FZD5 gene.•The reduction of miR-378a-3p gene may lead to reduction of FZD5 gene expression.•The reduction of miR-369-3p gene expression leads to decreased FZD5 gene expression. In this study, we aimed to elucidate the crosstalk between the Wnt/β-catenin signaling pathway and colorectal cancer (CRC) associated with inflammatory bowel disease (IBD) using a bioinformatics analysis of putative common biomarkers and a systems biology approach. The following criteria were used to search the GEO and ArrayExpress databases for terms related to CRC and IBD: 1. The dataset containing the transcriptomic data, and 2. Untreated samples by medications or drugs. A total of 42 datasets were selected for additional analysis. The GEO2R identified the differentially expressed genes. The genes involved in the Wnt signaling pathway were extracted from the KEGG database. Enrichment analysis and miRNA target prediction were conducted through the ToppGene online tool. In CRC datasets, there were 1168 up- and 998 down-regulated probes, whereas, in IBD datasets, there were 256 up- and 200 down-regulated probes. There were 65 upregulated and 57 downregulated genes shared by CRC and IBD. According to KEGG, there were 166 genes in the Wnt pathway. FriZZled5 (FZD5) was a down-regulated gene in both CRC and IBD, as determined by the intersection of CRC- and IBD-related DEGs with the Wnt pathway. It was also demonstrated that miR-191, miR-885-5p, miR-378a-3p, and miR-396-3p affect the FriZZled5 gene expression. It is possible that increased expression of miR-191 and miR-885-5p, or decreased expression of miR-378a -3p and miR396-3, in IBD and CRC results in decreased expression of the FZD5 gene. Based on the function of this gene, FZD5 may be a potential therapeutic target in IBD that progresses to CRC.