Proteomic Approach to FcεRI Aggregation-Initiated Signal Transduction Cascade in Human Mast Cells

• Published in: International archives of allergy and immunology Vol. 149; no. Suppl 1; pp. 73 - 76
• Main Authors: Yamaoka, Kazuko, Okayama, Yoshimichi, Kaminuma, Osamu, Katayama, Kazufumi, Mori, Akio, Tatsumi, Hideki, Nemoto, Sohichi, Hiroi, Takachika
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Basel, Switzerland Karger 01.01.2009
• Subjects: Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immunopathology; Medical sciences; Original Paper; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Mast cell; FcεRI; Phosphotyrosine; Proteomics; Aggregation; Human; Immunopathology; Signal transduction; Immunology; FcεRI receptor; Immunoglobulin receptor
• ISBN: 9783805591867; 3805591861
• ISSN: 1018-2438; 1423-0097
• DOI: 10.1159/000211376

Background: Mast cells (MCs) play a central role in allergic reactions through high-affinity IgE receptor (FcεRI)-mediated responses. Many attempts have been performed to investigate MC functions, though molecular bases of the intracellular signaling cascade through FcεRI, especially in human MCs, remain scant and unexplored. Methods: Human MCs were differentiated from CD34+ cells by culture with stem cell factor, IL-6 and IL-3. The differential phosphorylation profiles of protein tyrosine residues in the resulting MCs with or without FcεRI aggregation were examined by two-dimensional gel electrophoresis. The candidate phosphoproteins of interest were picked, in-gel digested and mass spectrometry fingerprinted. Results: Approximately 40 proteins in MCs were phosphorylated on their tyrosine residues in response to activation and some of them were identified. Particularly IL-31 receptor α, solute carrier family 39, syntaxin 5 and heterogeneous nuclear ribonucleoprotein are newly identified as phosphoproteins that are potentially involved in the MC signaling cascade through FcεRI. Conclusion: Our present phosphoproteome data may provide the clue to understand the molecular mechanisms for the activation of human MCs.