Physio-biochemical and DNA methylation analysis of the defense response network of wheat to drought stress

• Published in: Plant physiology and biochemistry Vol. 209; p. 108516
• Main Authors: Naderi, Salehe, Maali-Amiri, Reza, Sadeghi, Leila, Hamidi, Aidin
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.04.2024
• Subjects: Drought; MSAP; Oxidative stress; Tolerance responses; Wheat landrace; MSAP; Oxidative stress; Wheat landrace; Drought; Tolerance responses
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2024.108516

In the present work, physio-biochemical and DNA methylation analysis were conducted in wheat (Triticum aestivum L.) cultivars “Bolani” (drought-tolerant) and “Sistan” (drought-sensitive) during drought treatments: well-watered (at 90% field capacity (FC)), mild stress (at 50% FC, and severe stress (at 25% FC). During severe stress, O2•− and H2O2 content in cultivar Sistan showed significant increase (by 1.3 and 2.5-fold, respectively) relative to cultivar Bolani. In Bolani, the increased levels of radical scavenging activity (by 32%), glycine betaine (GB) (by 11.44%), proline (4-fold), abscisic acid (by 63.76%), and more stability of relative water content (RWC) (2-fold) were observed against drought-induced oxidative stress. Methylation level significantly decreased from 70.26% to 60.64% in Bolani and from 69.06% to 59.85% in Sistan during stress, and higher decreased tendency was related to CG and CHG in Bolani but CG in Sistan under severe stress. Methylation patterns showed that the highest polymorphism in Bolani was mainly as CG. As the intensity of stress increased, the enhanced physio-biochemical responses of Bolani cultivar were accompanied by a more decrease in the number of unchanged bands. According to heat map analysis, the highest difference (84.38%) in methylation patterns was observed between control and severe stress. Multivariate analysis using principal component analysis (PCA) showed a cultivar-specific methylation during stress and that methylation changes between cultivars are much higher than that of within a cultivar. Higher methylation to demethylation in Bolani (30.06 vs. 22.12%) compared to that of cultivar Sistan (23.21 vs. 30.15%) indicated more demethylation did not induce tolerance responses in Sistan. Sequencing differentially methylated fragments along with qRT-PCR analysis showed the efficient role of various DNA fragments, including demethylated fragments such as phosphoenol pyruvate carboxylase (PEPC), beta-glucosidase (BGlu), glycosyltransferase (GT), glutathione S-transferase (GST) and lysine demethylase (LSD) genes and methylated fragments like ubiquitin E2 enzyme genes in the development of drought tolerance. These results suggested the specific roles of DNA methylation in development of drought tolerance in wheat landrace. •DNA methylation links drought tolerance of wheat to metabolic acclimation.•During drought, methylation levels of Bolani outpaced demethylation and in Sistan the opposite happens.•The decreased tendency in methylation level was higher under severe stress compared to mild stress.•Drought tolerance was related to DNA methylation inducing positive/negative regulation of genes transcriptional activity.