Gallocyanine as a Fluorogen for the Identification of NADPH-Dependent Production of Superoxide Anion Radical by Blood Cells

• Published in: Russian journal of bioorganic chemistry Vol. 47; no. 1; pp. 299 - 306
• Main Authors: Panasenko, O. M., Reut, V. E., Borodina, I. V., Matyushkina, D. S., Ivanov, V. A., Grigorieva, D. V., Gorudko, I. V., Sokolov, A. V., Cherenkevich, S. N.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 2021; Springer Nature B.V
• Subjects: Anions; Antioxidants; Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Blood cells; Chemical sensors; Chemoreceptors; Fluorescence; Life Sciences; Mass spectrometry; Neutrophils; Organic Chemistry; Oxidation; Spectrofluorimetry; Spectrophotometry; Xanthine oxidase; reactive halogen species; superoxide anion radical; hypochlorous acid; gallocyanine; fluorescence; reactive oxygen species; oxidative stress
• ISSN: 1068-1620; 1608-330X
• DOI: 10.1134/S1068162021010179

— The interaction of the oxazine dye gallocyanine with reactive oxygen ( Н 2 О 2 ) and halogen (HOCl) species has been studied by spectrophotometry, mass spectrometry, and spectrofluorimetry. It has been shown that gallocyanine reacts with HOCl and (but not with Н 2 О 2 ) to form fluorescent products. It has been found using the inhibition assay that, both in the xanthine/xanthine oxidase system and in activated human blood neutrophils, the main contribution to the conversion of gallocyanine to a fluorophore comes from its reaction with The results obtained suggest that gallocyanine can act as a fluorogenic chemosensor and be used for estimating the activation of neutrophils and the NADPH-dependent production of superoxide anion radical by neutrophils and other blood cells, as well as for testing antioxidant drugs designed with the aim to correct diseases associated with oxidative stress.