SIMULTANEOUS DETERMINATION OF RUTIN AND BENZOCAINE IN SUPPOSITORIES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

A simple and precise reversed-phase HPLC procedure for the simultaneous determination of rutin and benzocaine in suppositories is proposed. The chromatographic conditions were: Apex ODS column; methanol-10 mM triethylamine aqueous solution pH 3.0 (57 : 43, v/v) as the mobile phase; detection wavelen...

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Bibliographic Details
Published inAnalytical letters Vol. 34; no. 15; pp. 2685 - 2692
Main Authors Joseph-Charles, J., Montagut, M., Langlois, M.-H., Boyer, C., Dubost, J.-P.
Format Journal Article
LanguageEnglish
Published Philadelphia, PA Taylor & Francis Group 31.12.2001
Taylor & Francis
Subjects
Analysis
Benzocaine
Biological and medical sciences
General pharmacology
High-performance liquid chromatography
Medical sciences
Pharmacology. Drug treatments
Rutin
Simultaneous determination
Suppositories
Local anesthetic
Benzocaine
Reversed phase chromatography
Chemical analysis
Multicomponent analysis
Ultraviolet detector
HPLC chromatography
Dosage form
Venous incompetence
Isocratic condition
Quantitative analysis
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Summary:A simple and precise reversed-phase HPLC procedure for the simultaneous determination of rutin and benzocaine in suppositories is proposed. The chromatographic conditions were: Apex ODS column; methanol-10 mM triethylamine aqueous solution pH 3.0 (57 : 43, v/v) as the mobile phase; detection wavelength, 270 nm. Calibration curves were linear at concentration ranges 6.0 μg ml −1 -14.0 μg ml −1 and 1.5 μg ml −1 - 3.5 μg ml −1 for rutin and benzocaine, respectively. The reproductibility was 1.05% and 0.95% for rutin and benzocaine, respectively.
ISSN:0003-2719
1532-236X
DOI:10.1081/AL-100108414