A serum-free medium for hybridoma cell culture

The effects of several different substances, including insulin, transferrin, ethanolamine, selenite and butyrate on the growth of murine hybridoma 2F7 cells, which secrete monoclonal antibody against small cell lung cancer, were investigated, and a serum-free medium SFM I was formulated. The effects...

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Bibliographic Details
Published inCytotechnology (Dordrecht) Vol. 11; no. 3; p. 169
Main Authors Chen, Z, Ke, Y, Chen, Y
Format Journal Article
LanguageEnglish
Published United States 1993
Subjects
Animals
Antibodies, Monoclonal
Butyrates - pharmacology
Butyric Acid
Carcinoma, Small Cell - immunology
Cell Division - drug effects
Culture Media, Serum-Free
Drug Synergism
Ethanolamine
Ethanolamines - pharmacology
Hybridomas - cytology
Hybridomas - drug effects
Hybridomas - immunology
Insulin - pharmacology
Lung Neoplasms - immunology
Mice
Serum Albumin, Bovine - pharmacology
Sodium Selenite - pharmacology
Transferrin - pharmacology
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Summary:The effects of several different substances, including insulin, transferrin, ethanolamine, selenite and butyrate on the growth of murine hybridoma 2F7 cells, which secrete monoclonal antibody against small cell lung cancer, were investigated, and a serum-free medium SFM I was formulated. The effects of taurine, spermidine, progesterone and adenine on the cell growth were tested further on the basis of the medium SFM I, and a modified serum-free medium SFM II was established. On the basis of medium SFM II, the substitution tests of ferric citrate for transferrin were carried out, and it was found that transferrin could be replaced. The experiments suggested that the formulated serum-free medium was suitable for 2F7 cell growth and monoclonal antibody secretion, and thus facilitated subsequent purification of monoclonal antibody.
ISSN:0920-9069
1573-0778
DOI:10.1007/BF00749866