Soluble Peroxidase in Fluid from the Intercellular Spaces of Tobacco Leaves

A high proportion of the soluble peroxidase of tobacco (Nicotiana tabacum L. var. Bottom Special) leaves is found in the fluid obtained by centrifugation of a buffer solution previously infiltrated into the intercellular spaces. Only a very small amount of the cytoplasmic enzyme, glucose 6-phosphate...

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Published inPlant physiology (Bethesda) Vol. 53; no. 2; pp. 317 - 318
Main Authors W. G. Rathmell, Sequeira, Luis
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Physiologists 01.02.1974
Subjects
Centrifugation
Dehydrogenases
Enzymes
Extracellular space
Leaves
Phytopathology
Plant cells
Short Communications
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Summary:A high proportion of the soluble peroxidase of tobacco (Nicotiana tabacum L. var. Bottom Special) leaves is found in the fluid obtained by centrifugation of a buffer solution previously infiltrated into the intercellular spaces. Only a very small amount of the cytoplasmic enzyme, glucose 6-phosphate dehydrogenase, is present in this fluid. Specific activity data suggest that an active process is responsible for the transfer of soluble peroxidase to the intercellular space and that the intercellular fluid fraction is not simply composed of material moving out of leaf cells by diffusion. The centrifugation method is a satisfactory means of isolating diluted intercellular fluid for biochemical and physiological investigations.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0032-0889
1532-2548
DOI:10.1104/pp.53.2.317