BrdU does not induce hepatocellular damage in experimental Wistar rats

Bromodeoxyuridine (BrdU) is used in studies related to cell proliferation and neurogenesis. The multiple intraperitoneal injections of this molecule could favor liver function profile changes. In this study, we evaluate the systemic and hepatocellular impact of BrdU in male adult Wistar rats in 30 %...

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Published inActa histochemica Vol. 126; no. 1; p. 152117
Main Authors Barrientos-Bonilla, Abril Alondra, Pensado-Guevara, Paola Belem, Puga-Olguín, Abraham, Nadella, Rasajna, Sánchez-García, Aurora del Carmen, Zavala-Flores, Laura Mireya, Villanueva-Olivo, Arnulfo, Cibrián-Llanderal, Iliana Tamara, Rovirosa-Hernández, María de Jesús, Hernandez-Baltazar, Daniel
Format Journal Article
LanguageEnglish
Published Germany Elsevier GmbH 01.01.2024
Subjects
Adult rats
Cell death
Hepatocellular damage
Intraperitoneal
Liver function
Intraperitoneal
Adult rats
Liver function
Hepatocellular damage
Cell death
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Summary:Bromodeoxyuridine (BrdU) is used in studies related to cell proliferation and neurogenesis. The multiple intraperitoneal injections of this molecule could favor liver function profile changes. In this study, we evaluate the systemic and hepatocellular impact of BrdU in male adult Wistar rats in 30 %-partial hepatectomy (PHx) model. The rats received BrdU 50 mg/Kg by intraperitoneal injection at 0.5, 1, 2, 3, 6, 9 and 16 days after 30 %-PH. The rats were distributed into four groups as follows, control, sham, PHx/BrdU(-) and PHx/BrdU(+). On day 16, we evaluated hepatocellular nuclei and analyzed histopathological features by haematoxylin-eosin stain and apoptotic profile was qualified by caspase-3 presence. The systemic effect was evaluated by liver markers such as alanine transferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), alkaline phosphatase (AP), bilirubin, total proteins and serum albumin content. The statistical analysis consisted of a student t-test and one-way ANOVA. BrdU did not induce apoptosis or hepatocellular damage in male rats. Multiple administrations of BrdU in male rats did not induce significant decrease body weight, but increased serum ALT and LDH levels were found. Our results show that the BrdU does not produce hepatocellular damage.
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ISSN:0065-1281
1618-0372
DOI:10.1016/j.acthis.2023.152117