Interaction of Escherichia coli RNA Polymerase with Artificial Promoters, Containing Nonnucleotide Spacers

To study the functional role of the spacer region between two consensus −10 and −35 elements of promoters, recognized by E. coli RNA polymerase, the model promoter-like DNA duplexes containing nonnucleotide inserts (mimicking 17-mer spacer) either in one or both strands, were constructed. The modifi...

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Published inNucleosides, nucleotides & nucleic acids Vol. 30; no. 7-8; pp. 667 - 679
Main Authors Koroleva, Olga Nikolaevna, Volkov, Eugeniy Mikhailovich, Drutsa, Valeriy Lvovich
Format Journal Article
LanguageEnglish
Published United States Taylor & Francis Group 01.07.2011
Subjects
Base Sequence
DNA - chemistry
DNA - metabolism
DNA-Directed RNA Polymerases - metabolism
E. coli RNA polymerase
Escherichia coli - enzymology
modified oligonucleotides
Molecular Sequence Data
Oligonucleotides - chemistry
Oligonucleotides - metabolism
Promoter Regions, Genetic
promoters
Protein Binding
Transcription, Genetic
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Summary:To study the functional role of the spacer region between two consensus −10 and −35 elements of promoters, recognized by E. coli RNA polymerase, the model promoter-like DNA duplexes containing nonnucleotide inserts (mimicking 17-mer spacer) either in one or both strands, were constructed. The modified duplexes can form the heparin-resistant binary complexes with RNA polymerase. The DNA duplex with nonnucleotide insert in the template strand can specifically direct the synthesis of mRNA in the in vitro run-off transcription assays.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1525-7770
1532-2335
DOI:10.1080/15257770.2011.598490