Glycophorin variants and Plasmodium falciparum: protective effect of the Dantu phenotype in vitro

Experimental work was carried out to establish the growth characteristics of Plasmodium falciparum in an in vitro culture system using cells with the Dantu, Henshaw and S-s-U- blood-group variants. A flow cytometric technique, using the dye thiazole orange, was adapted for use on the Epics Profile I...

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Published inHuman genetics Vol. 93; no. 2; p. 148
Main Authors Field, S P, Hempelmann, E, Mendelow, B V, Fleming, A F
Format Journal Article
LanguageEnglish
Published Germany 01.02.1994
Subjects
African Continental Ancestry Group - genetics
Animals
Cells, Cultured
Erythrocytes - parasitology
Flow Cytometry
Glycophorin - genetics
Humans
Phenotype
Plasmodium falciparum - growth & development
Rh-Hr Blood-Group System - genetics
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Summary:Experimental work was carried out to establish the growth characteristics of Plasmodium falciparum in an in vitro culture system using cells with the Dantu, Henshaw and S-s-U- blood-group variants. A flow cytometric technique, using the dye thiazole orange, was adapted for use on the Epics Profile II flow cytometer to count the parasites. This was performed at 24, 48 and 72 h. The ability of the parasites to grow in red cells of the Dantu and Henshaw phenotypes was also assayed by 3[H] hypoxanthine incorporation. Relative to control red cells, S-s-U- cells and Dantu cells were less suitable as host cells for P. falciparum in vitro. In contrast, cells expressing the Henshaw antigen were equally sensitive to P. falciparum infection as were normal controls. These data support the notion that glycophorins play an important role in P. falciparum infection. Further studies are required to evaluate the epidemiological significance of these results.
ISSN:0340-6717
DOI:10.1007/BF00210600