Antigenic relationships within the genus Salmonella as revealed by anti-Salmonella enteritidis monoclonal antibodies

• Published in: Veterinary research communications Vol. 26; no. 3; pp. 179 - 188
• Main Authors: Malik, M, Butchaiah, G, Bansal, M P, Siddiqui, M Z, Bakshi, C S, Singh, R K
• Format: Journal Article
• Language: English
• Published: Netherlands Springer Nature B.V 01.04.2002
• Subjects: Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - immunology; Antibody Specificity; Antigenic Variation - immunology; Antigens, Bacterial - immunology; Bacterial Outer Membrane Proteins - immunology; Blotting, Western; Enzyme-Linked Immunosorbent Assay; Mice; Mice, Inbred BALB C; Salmonella; Salmonella - classification; Salmonella - immunology; Salmonella enteritidis - immunology; Salmonella Infections - immunology
• ISSN: 0165-7380; 1573-7446
• DOI: 10.1023/A:1015249520170

A panel of 38 monoclonal antibodies (MAbs) that react with outer membrane proteins (OMPs) of Salmonella enteritidis was produced. On the basis of their binding pattern in ELISA, the MAbs were divided into three groups. The first group, consisting of 15 MAbs, was found to be Salmonella-specific as they did not cross-react with Escherichia coli or Pasteurella multocida. The second group of 15 MAbs cross-reacted with E. coli but not with P. multocida, reflecting the closer antigenic relationship of E. coli with Salmonella. The third group of 8 MAbs cross-reacted with both E. coli and P. multocida, indicating that the antigenic determinants identified by these MAbs are conserved in all the three genera. The antigenic relationship of the Salmonella serovars (S. enteritidis, S. gallinarum, S. typhimurium, S. dublin, S. agona, S. indiana and S. choleraesuis) was studied using OMPs prepared from them and the anti-S. enteritidis MAbs. Three MAbs appeared to be specific for S. enteritidis as they did not cross-react with any of the other Salmonella serovars. Twelve of the 38 MAbs cross-reacted with all the serovars tested. Six of these were specific to the Salmonella genus as they did not cross-react with any of the other Gram-negative bacteria tested. The reactivity pattern of the other MAbs indicated that S. gallinarum was antigenically close to S. enteritidis, followed in order by S. dublin, S. agona, S. typhimurium and S. indiana, whereas S. choleraesuis seemed to be antigenically quite distant from S. enteritidis.