Tumor cell loaded thermosensitive hydrogel for photodynamic therapy associated tumor antigens release

• Published in: Biochimica et biophysica acta. General subjects Vol. 1868; no. 11; p. 130703
• Main Authors: Khaliq, Nisar Ul, Lee, Juyeon, Kim, Yejin, Kim, Joohyeon, Kim, Taeho, Yu, Sohyeon, Seo, Dongseong, Sung, Daekyung, Kim, Hyungjun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2024
• Subjects: Animals; Antigens, Neoplasm - immunology; Antigens, Neoplasm - metabolism; Cell Line, Tumor; Gelatin; Humans; Hydrogel; Hydrogels - chemistry; Immunotherapy; Immunotherapy - methods; Mice; Neoplasms - drug therapy; Neoplasms - immunology; Neoplasms - metabolism; Neoplasms - pathology; Neoplasms - therapy; Photochemotherapy - methods; Photodynamic; Photosensitizing Agents - pharmacology; Pluronic® F-127; Poloxamer - chemistry; Reactive Oxygen Species - metabolism; PBS; SCMC; PDT; Photodynamic; DAPI; MTT; ROI; Pluronic® F-127; UV–vis; SDS-PAGE; RNO; Gelatin; Immunotherapy; NIR; ROS; DCF-DA; SEM; Hydrogel
• ISSN: 0304-4165; 1872-8006; 1872-8006
• DOI: 10.1016/j.bbagen.2024.130703

Background: Immunotherapy is a powerful strategy for treating cancer and can be used to inhibit the post-surgical relapse of tumors. Methods: To achieve this, a Cell@hydrogel was developed as a template using a mixture of CT26 tumor cells and Pluronic® F-127/gelatin. Results: The proposed mixture has a solution-to-gelation functionality and vice versa. The morphology of the Cell@hydrogel was characterized by scanning electron microscopy and confocal microscopy. For photodynamic immunotherapy, the Cell@hydrogel was functionalized with Cy7 (Cy7-Cell@hydrogel) to quantify reactive oxygen species in CT26 tumor cells. Gel electrophoresis and membrane integrity tests were performed to determine the efficiency of the Cy7-Cell@hydrogel following photodynamic therapy. Conclusions: This protocol provides an alternative approach that mechanistically inhibits the post-surgical relapse of solid tumors based on immunotherapy. [Display omitted] •Cell@hydrogel was developed using CT26 tumor cells and Pluronic® F-127/gelatin.•Scanning electron microscopy and confocal microscopy suggested morphology.•Cy7-Cell@hydrogel exhibited significant Photothermal and photodynamic performances.•Invitro cell assays revealed inhibition of relapse of post-surgical solid tumors.