Determination of 20(S)-ginsenoside Rh1 and its aglycone 20(S)-protopanaxatriol in rat plasma by sensitive LC-APCI-MS method and its application to pharmacokinetic study

• Published in: European journal of mass spectrometry (Chichester, England) Vol. 15; no. 1; p. 57
• Main Authors: Lai, Li, Liu, Yitong, Hao, Haiping, Wang, Guangji, Chen, Xijing, Ren, Hongcan
• Format: Journal Article
• Language: English
• Published: England 01.01.2009
• Subjects: Animals; Atmospheric Pressure; Blood Chemical Analysis - methods; Blood Chemical Analysis - statistics & numerical data; Chromatography, Liquid - methods; Chromatography, Liquid - statistics & numerical data; Ginsenosides - blood; Ginsenosides - chemistry; Ginsenosides - pharmacokinetics; Mass Spectrometry - methods; Mass Spectrometry - statistics & numerical data; Molecular Structure; Rats; Rats, Sprague-Dawley; Sapogenins - blood; Sapogenins - chemistry; Sapogenins - pharmacokinetics; Sensitivity and Specificity
• ISSN: 1469-0667
• DOI: 10.1255/ejms.974

Liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (LC-APCI-MS) method has been developed for the measurement of the concentrations of 20(S)-ginsenoside Rh1 and its aglycone 20(S)-protopanaxatriol in rat plasma with panaxatriol as internal standard. The method involved single liquid-liquid extraction of both 20(S)-ginsenoside Rh1 and 20(S)-protopanaxatriol from plasma samples with n-butanol. The limit of quantification (LOQ) was 5 ng mL(-1) for both compounds. The method was validated within the linear range 5-2000 ng.mL(-1) for both compounds. The correlation coefficient for the calibration regression line was 0.999 or better. Intra-day and inter-day accuracy were better than 15%. The method has been successfully used for the pharmacokinetic studies in rats. After intravenous administrations, the mean retention times of 20(S)-ginsenoside Rh1 and 20(S)-protopanaxatriol were 17.1 +/- 2.0 min and 3.46 +/- 0.33 h, respectively.