Demonstration of multiple forms of bovine brain myristoyl CoA:protein N-myristoyl transferase

• Published in: Molecular and cellular biochemistry Vol. 113; no. 1; p. 77
• Main Authors: King, M J, Sharma, R K
• Format: Journal Article
• Language: English
• Published: Netherlands 06.07.1992
• Subjects: Acyltransferases - isolation & purification; Animals; Brain - enzymology; Cattle; Chromatography, Ion Exchange; Isoenzymes - isolation & purification; Kinetics; Molecular Weight; Substrate Specificity

Four distinct N-myristoyl transferase (NMT) activity peaks, designated I, II, III, and IV, were separated from the cytosolic fraction of bovine brain by DEAE-Sepharose column chromatography. Peaks I, II, III and IV were characterised biochemically with respect to substrate specificity: with cAMP-dep...