Demonstration of multiple forms of bovine brain myristoyl CoA:protein N-myristoyl transferase

Four distinct N-myristoyl transferase (NMT) activity peaks, designated I, II, III, and IV, were separated from the cytosolic fraction of bovine brain by DEAE-Sepharose column chromatography. Peaks I, II, III and IV were characterised biochemically with respect to substrate specificity: with cAMP-dep...

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Bibliographic Details
Published inMolecular and cellular biochemistry Vol. 113; no. 1; p. 77
Main Authors King, M J, Sharma, R K
Format Journal Article
LanguageEnglish
Published Netherlands 06.07.1992
Subjects
Acyltransferases - isolation & purification
Animals
Brain - enzymology
Cattle
Chromatography, Ion Exchange
Isoenzymes - isolation & purification
Kinetics
Molecular Weight
Substrate Specificity
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Summary:Four distinct N-myristoyl transferase (NMT) activity peaks, designated I, II, III, and IV, were separated from the cytosolic fraction of bovine brain by DEAE-Sepharose column chromatography. Peaks I, II, III and IV were characterised biochemically with respect to substrate specificity: with cAMP-dependent protein kinase and pp60src derived peptides, and by their apparent molecular mass. The apparent molecular mass of peaks I, II, III and IV were 190 kDa, 224 kDa, 390 kDa and 76 kDa, respectively. These results indicate that bovine brain contains multiple forms of NMT.
ISSN:0300-8177
DOI:10.1007/BF00230888