Succinoyl trehalose lipid induced differentiation of human monocytoid leukemic cell line U937 into monocyte-macrophages

• Published in: Cytotechnology (Dordrecht) Vol. 19; no. 1; p. 79
• Main Authors: Isoda, H, Shinmoto, H, Matsumura, M, Nakahara, T
• Format: Journal Article
• Language: English
• Published: United States 1995
• Subjects: Biomarkers; Carbohydrate Conformation; Carcinoma - pathology; Cell Differentiation - drug effects; Cell Line; Fatty Acids - pharmacology; Glycolipids - pharmacology; Humans; Lung - cytology; Lung Neoplasms - pathology; Lymphoma, Large B-Cell, Diffuse - pathology; Macrophages; Molecular Structure; Monocytes; Neoplastic Stem Cells - cytology; Neoplastic Stem Cells - drug effects; Phagocytosis - drug effects; Rhodococcus - metabolism; Sodium Dodecyl Sulfate - pharmacology; Succinates - pharmacology; Succinic Acid; Trehalose - pharmacology; Tumor Cells, Cultured - drug effects
• ISSN: 0920-9069; 1573-0778
• DOI: 10.1007/BF00749758

A novel type of succinoyl trehalose lipid (STL-1) prepared from n-hexadecane-culture of Rhodococcus erythropolis SD-74 markedly inhibited the growth of a human monocytoid leukemic cell line, U937, and induced its morphological alteration along a monocyte-macrophage lineage. STL-1 markedly increased differention-associated characteristics in macrophage, such as nitroblue tetrazolium reducing ability, appearance of Fc receptor, phagocytic activities in U937. Furthermore, U937 cells, which were activated with STL-1 exhibited cytotoxic activity against human lung carcinoma cell line A549. However, STL-1 did not affect growth of a normal human fetal lung cell line TIG-1. The individual components of STL-1, neither sugar moiety nor fatty acids in the free form, were effective at inducing the differentiation of U937 cell. From these results, we concluded that STL-1 has low cytotoxicity against normal human cells and the ester molecule itself is responsible for the activity of inducing differentiation of human monocytoid leukemic cell line U937 into monocyte-macrophage which results in the stimulation of the production of some cytotoxic substances.